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The simultaneous oxidation and phosphorylation of glyceraldehyde 3-phosphate forms a highly reactive covalent thioester bond between a cysteine side chain (reactive group ?SH) on the enzyme (glyceraldehyde 3-phosphate dehydrogenase) and the oxidized intermediate (see the arrow in Figure 13-23A) .If the enzyme had a serine (reactive group ?OH) instead of a cysteine at this position, which could form only a much lower energy bond to the oxidized substrate (see the arrow in Figure 13-23B) , how might this new enzyme act? (A)
(B)
Figure 13-23
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