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If the Tn10 Transposon Is Used to Mutagenize a Bacterium

question 7

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If the Tn10 transposon is used to mutagenize a bacterium for the purpose of creating a mutation in the his operon, what would be the most appropriate selection media?

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Definitions:

Consanguineous Matings

Reproductive relationships between individuals who are closely related genetically, often leading to an increase in the probability of their offspring inheriting genetic disorders.

Autosomal Recessive Alleles

Alleles of a gene located on an autosome that need to be present in two copies (homozygous) in order for a trait or disorder to manifest.

Genetic Screening

A systematic search through a population for individuals with a genotype or karyotype that might cause a serious genetic disease in them or their offspring.

Newborns

Very young babies, often defined as being within the first hours to several weeks of life.

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