In Sanger Sequencing, What Causes DNA Synthesis to Terminate at a Specific

question 16

Multiple Choice

In Sanger sequencing, what causes DNA synthesis to terminate at a specific base?

• A)Restriction endonucleases cleave DNA after particular bases.
• B)Fluorescent molecules produce high-energy light that inactivates DNA polymerase.
• C)Incorporation of a deoxynucleotide that lacks a base causes DNA polymerase to stall.
• D)Incorporation of a dideoxynucleotide that lacks a 3′ hydroxyl.
• E)Incorporation of a ribonucleotide that lacks a 5' phosphate.

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