You Performed a Sanger Sequencing Reaction and Obtained the Following

question 12

Multiple Choice

You performed a Sanger sequencing reaction and obtained the following read.In this figure, A = green, C = purple, G = black, T = red.The height of the peaks is unimportant.The smallest molecule is at the left of the trace.
-Which is a step in hierarchical genome sequencing that is not found in shotgun genome sequencing?

• A)fragment the genome
• B)construct a genomic library
• C)organize the clones based on their order in the genome
• D)sequence the ends of millions of clones
• E)assemble the sequences into contigs based on overlap

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