A Researcher Is Interested in Testing the Role for a Highly-

question 12

Multiple Choice

A researcher is interested in testing the role for a highly- conserved amino acid in the function of a mouse protein. She has been unable to locate any pre- existing variants in this codon among a large number of available mouse strains. What might be her approach to generating such mutants?

• A) Use RNAi to knockdown the expression of the target gene.
• B) Use RNAi to interfere with translation of the gene.
• C) Use transposons to generate insertion alleles in the gene.
• D) Measure the expression of this gene in mice as they develop.
• E) Use CRISPR- cas to cause DNA cleavage at the codon encoding this amino acid.

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