Identify the Correct Sequence in Which the Steps Below Occur

question 50

Multiple Choice

Identify the correct sequence in which the steps below occur during a single PCR cycle.
1) Complementary base pairing between primers and target DNA.
2) Addition of DNA nucleotides by Taq DNA polymerase.
3) Heat separation of strands of target DNA.

• A) 3; 1; 2
• B) 1; 2; 3
• C) 1; 3; 2
• D) 2; 3; 1
• E) 2; 1; 3

---

Definitions:

Weighted-Average Method

An inventory costing approach that assigns a weighted average cost to each unit of inventory, used in valuing the cost of goods sold and ending inventory.

Process Costing System

An accounting method used to allocate costs to units of product in continuous processes or industries where the products are indistinguishable from each other.

Equivalent Unit

A measure used in cost accounting to represent the amount of work done on partial units of production, making them comparable to fully completed units.

Cost Reconciliation Report

A report that reconciles the total costs incurred with the costs accounted for in a specific period, used in cost accounting to ensure accuracy.