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In Isoelectric Focusing, Is the Low-PH End Close to the Anode

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In isoelectric focusing, is the low-pH end close to the anode (A; positive electrode) or cathode (C)? Would you use octylglucoside (O; a nonionic detergent) or SDS (S) for separation of proteins by this technique? Would a highly basic protein be near the high-pH (H) or low-pH (L) end of the strip at the end of the experiment? Write down your answer as a three-letter string using the letters in the parentheses above, e.g. ASH.


Definitions:

Impulse Buying

A spontaneous, unplanned decision to buy a product or service, typically made right before a purchase.

Neoclassical Theory

An economic theory that focuses on how the perception of utility and costs influences supply and demand in markets, leading to an equilibrium.

Impulse Buying

A spontaneous, unplanned decision to buy a product or service made just before a purchase.

Hedonic Treadmill

The theory that people perpetually return to a relatively stable level of happiness despite major positive or negative events or life changes.

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