Indicate Whether You Would Use a Fluorescent Organic Molecule (O)

question 20

Short Answer

Indicate whether you would use a fluorescent organic molecule (O), in situ hybridization (H), or a coupled fluorescent protein (P) to visualize the cells and their molecules in each of the following cases. Your answer would be a five-letter string composed of letters O, H, and P only, e.g. OHOOO.
( ) You would like to see where in the early Drosophila embryo the mRNA encoding a certain transcription regulator is located.
( ) You would like to see the nuclei and count them in an early mouse embryo.
( ) You would like to visualize chromosome 3 in a human cell culture derived from a patient's tissue, based on specific sequences present on this chromosome.
( ) You would like to observe the oscillations in Ca²? ions inside a fertilized frog egg.
( ) You would like to compare the localization of two transcription regulatory proteins in cultured human T cells.

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Definitions:

Compulsively

Acting driven by an irresistible urge, often against one's conscious wishes and in a repetitive, ritualistic manner.

Neurotic Trends

Psychological tendencies in an individual that lead to negative patterns of thought and behavior, often resulting from unresolved conflicts and anxieties.

Self-Protective Mechanisms

Psychological strategies, often unconscious, that individuals employ to defend themselves from perceived threats or to manage emotional pain.

Compulsive Attitudes

Mindsets characterized by an uncontrollable urge to perform certain actions or think in a particular way.