You Have Engineered a Fusion Protein Composed of a Cis

question 46

Short Answer

You have engineered a fusion protein composed of a cis Golgi resident protein and the green fluorescent protein. Similarly, you have made a fusion protein composed of a trans Golgi protein and the red fluorescent protein. You express these proteins in cells and follow individual Golgi cisternae using fluorescence microscopy. You observe that each individual cisterna emits a green, faint yellow, or red fluorescent signal at different times: it initially shows green fluorescence, but the green fluorescent signal fades away with time, and instead red fluorescence appears in the same cisterna. Additionally, you do not observe any red fluorescent cisterna that becomes green over time. Do these observations agree better with the cisternal maturation model (C) or the vesicle transport model (V) for the organization of Golgi stacks? Write down C or V as your answer.

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Definitions:

Family Group

A social unit consisting of people related by blood, marriage, or adoption, living together or maintaining close emotional ties.

Racial-Ethnic Intermarriage

Marriage between partners who belong to different racial or ethnic groups.

Ethnic Identity

A person's sense of belonging to a particular ethnic group, including the customs, culture, and perception of shared heritage or ancestry.

Middle Eastern

Pertaining to the geographical region that encompasses Western Asia and Egypt, noted for its distinct cultural, religious, and historical identity.