Real-Time PCR Can Be Used to Quantify the Original Amount

question 17

Multiple Choice

Real-time PCR can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application?

• A) careful design of specific forward and reverse primers
• B) RNA must first be converted to cDNA
• C) RNA must be denatured with formaldehyde to avoid secondary structures
• D) separation of mRNA from other RNA species
• E) none of the above

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