Refer to the figure, which shows a phylogenetic tree of the engrailed gene family. 
Which gene is the most closely related ortholog of human En1?
Definitions:
Nonindustrialized Country
A country with less economic development and lower living standards compared to industrialized nations.
Regular Medical Checkups
Routine examinations by a healthcare professional to assess health status and prevent future illnesses.
Maturation
The process of development and growth, typically referring to physical, psychological, or emotional development towards maturity.
Sanitation
The processes and practices used to maintain a clean, hygienic, and healthy living environment.
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