If Negatively Supercoiled Plasmid DNA Is Mixed with Human Topoisomerase

question 1

Multiple Choice

If negatively supercoiled plasmid DNA is mixed with human topoisomerase I and ATP, which effect would be observed?

• A) Rapid ATP hydrolysis would occur.
• B) The linking number for the plasmid would increase.
• C) The writhe on the plasmid would increase.
• D) The superhelical density would become more negative.
• E) None of these effects would be observed.

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Definitions:

Permanganate

A powerful oxidizing agent, often encountered as potassium permanganate (KMnO4), used in various chemical reactions and as a disinfectant.

Ozone

A molecule composed of three oxygen atoms (O3) known for its protective layer in the Earth's stratosphere and as a pollutant at ground level.

Phenylacetic Acid

An organic compound with the formula C6H5CH2COOH, used in the synthesis of various pharmaceuticals.

Grignard Chemistry

A branch of organic chemistry focusing on the reactions involving Grignard reagents, which are magnesium-based compounds used to form carbon-carbon bonds.