If Negatively Supercoiled Plasmid DNA Is Mixed with Human Topoisomerase

question 1

Multiple Choice

If negatively supercoiled plasmid DNA is mixed with human topoisomerase I and ATP, which effect would be observed?

• A) Rapid ATP hydrolysis would occur.
• B) The linking number for the plasmid would increase.
• C) The writhe on the plasmid would increase.
• D) The superhelical density would become more negative.
• E) None of these effects would be observed.

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Definitions:

Net Present Value

A financial metric that calculates the present value of all future cash flows associated with an investment, minus the initial investment cost.

Present Value

The current worth of a future sum of money or stream of cash flows given a specified rate of return.

Unequal Lives

A term used in capital budgeting to describe projects or investments with different durations.

Replacement Chain Method

A capital budgeting technique that compares different investment projects with unequal lifespans by calculating their equivalent annual cost.