If a FRET Experiment Was Carried Out with Blue Fluorescent $\alpha$

question 98

Multiple Choice

If a FRET experiment was carried out with blue fluorescent protein attached to the $\alpha$ subunit of a stimulatory G-protein and a green fluorescent protein attached to the $\beta$ subunit, the presence of a strong FRET signal would be associated with:

• A) interaction of a G protein-coupled receptor with an antagonist.
• B) hydrolysis of GTP in the \(\alpha\) subunit.
• C) increased concentrations of cAMP.
• D) both interaction of a G protein-coupled receptor with an antagonist and hydrolysis of GTP in the \(\alpha\)subunit.
• E) both hydrolysis of GTP in the \(\alpha\) subunit and increased concentrations of cAMP.

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