The AgeI Restriction Enzyme Recognizes the Sequence ACCGGT and Cuts

question 3

Multiple Choice

The AgeI restriction enzyme recognizes the sequence ACCGGT and cuts each strand between the first (A) and the second (C) nucleotide. This can be represented as A|CCGGT, with the vertical bar indicating the cleavage position. If a DNA fragment is cut with AgeI at both ends, cutting a plasmid with which of the following enzymes would then allow insertion of the fragment into the plasmid by simple ligation of sticky ends?

• A) FseI, with the recognition sequence GGCCGG|CC
• B) AatI, with the recognition sequence AGG|CCT
• C) BstZI, with the recognition sequence C|GGCCG
• D) MroI, with the recognition sequence T|CCGGA
• E) None of the above

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