In Analysis Using Two-Dimensional Gel Electrophoresis of the Proteins Expressed

question 34

Multiple Choice

In analysis using two-dimensional gel electrophoresis of the proteins expressed in different cell types, the number of spots that are different in different cells usually exceeds the number of common spots, and even the common spots can still have different intensities. The spots representing which of the following proteins would you expect to be among the common spots when compared across several cell types?

• A) RPL10 (a ribosomal protein)
• B) HBA1 (a hemoglobin subunit)
• C) Insulin (a hormone)
• D) Tyrosine aminotransferase (a metabolic enzyme)
• E) All of the above

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Definitions:

Chi-squared Test

A chi-squared test is a statistical method used to assess the differences between observed and expected frequencies in one or more categories.

Expected Values

The long-term average or mean value of a random variable over numerous trials or occurrences.

Null Hypothesis

A default hypothesis that indicates no effect or no difference, and against which the alternative hypothesis is tested.

Proportions

The ratio of part to whole, expressed as a fraction or percentage, describing the relative size of parts within a population or a whole.